Baking Soda (Sodium Bicarbonate) As An Alternative To Lithium Carbonate Blueing Agent In Hematoxylin-Eosin Staining

Burhanuddin Burhanuddin; Malik Abil Fadli; Neiny Prisy Foekh; Ayu Putri Utami

doi:10.62951/ijhsb.v1i4.156

Authors

Burhanuddin Burhanuddin Poltekkes Kemenkes Jakarta III
Malik Abil Fadli Poltekkes Kemenkes Jakarta III
Neiny Prisy Foekh Poltekkes Kemenkes Jakarta III
Ayu Putri Utami Poltekkes Kemenkes Jakarta III

DOI:

https://doi.org/10.62951/ijhsb.v1i4.156

Keywords:

Bluing, Lithium carbonate, Baking soda

Abstract

Hematoxylin-eosin staining is a crucial technique used in histopathology to differentiate tissue components. One of the steps in this staining is changing the color of the nucleus, which was previously reddish due to the acid solution, to bluish; this step is known as blueing. The bluing agent, lithium carbonate, is commonly used in laboratories but has side effects and low availability. Sodium bicarbonate, also known as baking soda, can be used as an alternative due to its optimal pH, lower toxicity, and availability. This study aims to find out whether baking soda is as effective as lithium carbonate when used as a blueing agent for hematoxylin-eosin staining. The study design used was a true experimental post-test only control group design with a sample size of 32 preparations. Results of preparations using baking soda microscopically had good color uniformity, the nucleus was visible, the cytoplasm was clear, so the boundaries between cells were visible, and no cell lysis was found. In percentage terms, 14 preparations received a score of two (87.5%), two preparations received a score of three (12.5%), followed by a P value of 0.051 after carrying out the Fisher's exact test in bivariate analysis. Based on the results, baking soda is as effective as lithium carbonate, so it can be used as an alternative to bluing agent of hematoxylin-eosin staining.

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References

Gitlin, M. (2016). Lithium side effects and toxicity: Prevalence and management strategies. International Journal of Bipolar Disorders, 4(1). https://doi.org/10.1186/s40345-016-0068-y

Khristian, E., & Inderiati, D. (2017). Cytohistotechnology (N. Fitriana & H. Junianto, Eds.). Ministry of Health of the Republic of Indonesia; Center for Health Education and Human Resources.

Ministry of Agriculture of the Republic of Indonesia. (2016). Use and handling of experimental animals (rodents) in research in accordance with animal welfare. Center for Animal Husbandry Research and Development.

Mohandas, R., Ramani, P., Sherlin, H. J., Gheena, S., Ramasubramanian, A., Jayaraj, G., Don, K. R., & Santhanam, A. (2019). Lithium carbonate as a bluing agent – A comparative study. Research Journal of Pharmacy and Technology, 12(10), 4895–4898. https://doi.org/10.5958/0974-360X.2019.00847.3

Pambudi, S. (2015). The effect of the proportion of baking soda (sodium bicarbonate) and powdered ammonia (ammonium bicarbonate) as a developing agent on the physical, chemical, and organoleptic properties of bagiak cake [Brawijaya University]. http://repository.ub.ac.id/id/eprint/150586

Salafudin, S. (2021). Indonesia's lithium natural resources. Journal of Green Engineering, 5(2), 178–187. https://doi.org/10.26760/jrh.v5i2.178-187

Sari, E. J. (2016). The structure of the fetal spine of mice (Mus musculus L.) after administration of nutmeg rhizome extract (Cyperus rotundus L.). Jurnal Biologi Eksperimen dan Kehidupan, 3(1). https://doi.org/10.23960/jbekh.v3i1.67

Wahyuni, W., Idris, F., Septiadi, M. G. S., Pitriani, P., & Susanto, C. (2020). Method verification: General histopathological staining analysis of modified hematoxylin and eosin for Negri bodies rabies. Agricultural Repository, 67–74. https://repository.pertanian.go.id/handle/123456789/11686

Wang, H., Jiang, C., Yang, Y., Li, J., Wang, Y., Wang, C., & Gao, Y. (2022). Resveratrol ameliorates iron overload-induced liver fibrosis in mice by regulating iron homeostasis. PeerJ, 10, 1–19. https://doi.org/10.7717/peerj.13592

Yusuf, M., Al-Gizar, M. R., Rorrong, Y. Y. A., Badaring, D. R., Aswanti, H., M. Z., S. M. A., Nurazizah, D., Dzalsabila, A., Ahyar, M., Wulan, W., Putri, M. J., & Arisma, W. F. (2022). Animal management and management techniques. In Biology Department, FMIPA UNM.